Plasmid

Part:BBa_K382000:Design

Designed by: Christina Agapakis   Group: iGEM10_Harvard   (2010-10-26)


pORE Open Series Vector with BioBrick MCS (Basta resistance)


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 4759
    Illegal XbaI site found at 4774
    Illegal SpeI site found at 4780
    Illegal PstI site found at 4794
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 4759
    Illegal SpeI site found at 4780
    Illegal PstI site found at 4794
    Illegal NotI site found at 4765
    Illegal NotI site found at 4787
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 4759
    Illegal BglII site found at 7193
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 4759
    Illegal suffix found in sequence at 4780
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 4759
    Illegal XbaI site found at 4774
    Illegal SpeI site found at 4780
    Illegal PstI site found at 4794
    Illegal NgoMIV site found at 625
    Illegal NgoMIV site found at 749
    Illegal NgoMIV site found at 2181
    Illegal NgoMIV site found at 2772
    Illegal NgoMIV site found at 6642
    Illegal AgeI site found at 341
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 6663


Design Notes

Conforms to Silver Assembly Standard 21


Source

Modified vector pORE_O1 from the Arabidopsis Information Resource

References